Indirizzo

Via MIcheli 3 - 50121 Firenze

Telefono

055 4574065

Esperienza lavorativa

• Date (da – a)

Settembre 2020-

• Nome e indirizzo del datore di lavoro

Dipartimento di Biologia

Universita’ di Firenze, Italia, 50121

• Tipo di impiego

Assistant Professor (Fixed Term) BIO/01. RTDA L.240/10

• Principali mansioni e responsabilità

Didattica, Ricerca e attivita’ progettuale ai fini di finanziamento.

• Date (da – a)

Marzo 2020-Agosto 2020

• Nome e indirizzo del datore di lavoro

Plant Biology

Michigan State University, East Lansing, MI 48824-1312

• Tipo di impiego

Assistant Professor (Fixed Term)

• Principali mansioni e responsabilità

Ricerca e attivita’ progettuale ai fini di finanziamento

• Date (da – a)

Settembre 2014-Febbraio 2020

• Nome e indirizzo del datore di lavoro

MSU-DOE Plant Research Laboratory

Michigan State University, East Lansing, MI 48824-1312

• Tipo di impiego

Assistant Professor (Fixed Term)

• Principali mansioni e responsabilità

Ricerca e attivita’ progettuale ai fini di finanziamento

• Date (da – a)

Settembre 2009-Settembre 2014

• Nome e indirizzo del datore di lavoro

MSU-DOE Plant Research Laboratory

Michigan State University, East Lansing, MI 48824-1312

• Tipo di impiego

Post-Doctoral Position

• Principali mansioni e responsabilità

Ricerca e attivita’ progettuale ai fini di finanziamento

• Date (da – a)

Marzo 2006-Agosto 2009

• Nome e indirizzo del datore di lavoro

Dipartimento di Ortofloro

Universita’ di Firenze, Italia, 50019

• Tipo di impiego

Assegnista di ricerca

• Principali mansioni e responsabilità

Ricerca e supporto sviluppo progettuale

• Date (da – a)

Dicembre 2002-Gennaio 2004

• Nome e indirizzo del datore di lavoro

• Tipo di impiego

Esperienza lavorativa post-laurea

• Principali mansioni e responsabilità

Ricerca

Istruzione e formazione

• Date (da – a)

06/11/2018 - 06/11/2029

• Principali materie / abilità professionali oggetto dello studio

05/A1 - BOTANICA

• Qualifica conseguita

ABILITAZIONE PROFESSORE I E II FASCIA 05/A1

• Date (da – a)

10/01/2020 AL 10/01/2029

• Principali materie / abilità professionali oggetto dello studio

05/E2 BIOLOGIA MOLECOLARE

• Qualifica conseguita

ABILITAZIONE PROFESSORE II FASCIA 05/E2

• Date (da – a)

Marzo 2006-Agosto 2009

• Nome e tipo di istituto di istruzione o formazione

Department of Biology, University of Bonn (Germany)

• Principali materie / abilità professionali oggetto dello studio

Plant Cell and Molecular Biology

• Qualifica conseguita

• Livello nella classificazione nazionale (se pertinente)

Dottorato di ricerca

Titolo della tesi

“Trans-Golgi Network as independent organelle from Golgi apparatus in plant cells ”.

• Date (da – a)

Gennaio 2004-Gennaio 2006

• Nome e tipo di istituto di istruzione o formazione

Department of Biology, University of Saskatchewan (Canada)

• Principali materie / abilità professionali oggetto dello studio

BIOL 825-Topics in Plant Molecular Biology;BIOL 832-Control of plant growth and development; BIOL 898-Plant Cell Structure; BIOL 990-Seminar; BIOL 994-Research

• Qualifica conseguita

• Livello nella classificazione nazionale (se pertinente)

Master di ricerca

Titolo della tesi

“AtArl1, a small GTPase and its role in the binding of the GRIP domain to the Golgi apparatus in plant cells”.

• Date (da – a)

Luglio 2003

• Nome e tipo di istituto di istruzione o formazione

Ministero dell’istruzione dell’Universita’ e della ricerca

• Principali materie / abilità professionali oggetto dello studio

Biologia

• Qualifica conseguita

Abilitazione alla professione di Biologo

• Date (da – a)

Ottobre 1996-Dicembre 2002

• Nome e tipo di istituto di istruzione o formazione

Universita’ del Salento (Italia).

• Principali materie / abilità professionali oggetto dello studio

Biologia cellulare e molecolare

• Qualifica conseguita

Laurea in Science Biologiche con indirizzo Bio-molecolare

Titolo della tesi

“Variazioni nel metabolismo del glucosio in cellule di tabacco durante lo stress ossidativo”.

Elementi di riferimento

Numero Registro :fg111n.1106

Capacità e competenze personali

Acquisite nel corso della vita e della carriera ma non necessariamente riconosciute da certificati e diplomi ufficiali.

Madrelingua

Italiano

Altre lingua

Inglese

Francese

ATTRIBUZIONE DI INCARICHI DI INSEGNAMENTO:

Dal 02/2023 al 06/2023: Botanica con laboratorio (BIO/01), Dipartimento di Biologia, Università di Firenze. (9CFU/72 ore insegnamento);

Dal 02/2022 al 06/2022: Botanica con laboratorio (BIO/01), Dipartimento di Biologia, Università di Firenze. (9CFU/72 ore insegnamento);

Dal 02/2021 al 06/2021: Botanica con laboratorio (BIO/01), Dipartimento di Biologia, Università di Firenze. (2CFU/24 ore insegnamento);

Dal 22-01-2015 al 22-01-2015: Incarico di docenza presso Michigan State University, Plant biology, 2015: MMG 409 (2 hours) Undergraduate Level. Dal 22-01-2015 al 22-01-2015

Dal 13-01-2011 al 13-01-2011: Incarico di docenza presso Michigan State University, Plant biology 2011. Graduate Level : PLB 800 (2 hours) Seminar in plant Biology

Dal 30-05-2008 al 30-05-2008: Incarico di docenza presso il Dipartimento di Plant, Soil and Environmental Science, Polo Scientifico, Sesto Fiorentino (Firenze). Corso per dottorandi (Tecnologie innovative) (2 CFU): "Basi di microscopia confocale" 2008. Dal 30-05-2008 al 30-05-2008

Teaching assistant (corso biologia di base con laboratori) per l`anno accademico 2005-2006 presso il dipartimento di Biologia, Università del Saskatchewan (Canada), attività di tutoraggio studenti durante ore di lezione, assistenza durante esami, correzione e valutazione degli elaborati.

FORMAZIONE PROFESSIONALE:

2019: Use of the transmission electron microscope (TEM) Jeol 1400 flash. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. November 2019.

2019: Light sheet microscopy. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. October 2019.

2019: Workshop PhotosynQ Hands-on Lead by Prof. David Kramer): how to operate and use the MultispeQ: 1. Overview of Applications and MultispeQ Fundamentals 2. Learn to navigate the apps and operate your instrument. 3. Project Generation and Experiment Design. 4. Live demonstration creating a project using existing protocols and best practices for experiment efficiencies and accuracy. 5. Hands on Data Collection 6. Actual field data collection in small teams. 7 Evaluation of data. 8.Live demonstration of analyses of real data sets using online PhotosynQ tools. April 2019.

2018: Strategies for teaching science practices using free technologies at MSU (Michigan State University), 13 Nov 2018.

2017: Write Winning Grant Proposals. John D. Robertson, Grant Writers' Seminars and Workshops, LLC.Presentor: John D. Robertson, Ph.D., associate member of the Grant Writers' Seminars and Workshops, LLC. Idea development, Identification of the most appropriate granting agency, How to write for reviewers, Tips and strategies that are of proven value in presenting an applicant's case to reviewers. January 2017.

2017: Conseguimento attestato di insegnamento. “Pathways to Scientific Teaching” (by Prof. Diane Ebert-May, Michigan State University) September-November 2016.

2016: Use of the transmission electron microscope (TEM) and preparative equipment in the biological sciences. Sectioning for electron microscopy. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. December 2016.

2015: Total Internal Reflection Fluorescence (TIRF) microscopy and Super Resolution STORM imaging usingNikon A1 CLSM. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. June 2015.

2014: Laser Capture Microscopy (LCM) using Zeiss Palm MicroBeam IV Laser Capture Microscope. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. February 2014.

2013: Workshop: Fluctuation cross-correlation analysis for the study of fluorescent reporters in tip growth (organized by Luis Vidali) University of Wisconsin, Madison, USA. June 2013.

2013:Workshop: Kinematics (organized by Wendy Silk, Edgar Spalding, and Nathan Miller) University of Wisconsin, Madison, USA. June 2013.

2012: Workshop: Image signal quantification (Kevin Eliceiri) University of Wisconsin, Madison, USA. June 2012.

2012: Workshop: Implementation of computational models: entry points and first steps (Anja Geitmann) University of Wisconsin, Madison, USA. June 2012.

2011: Fluorescence Correlation Spectroscopy using Zeiss LSM Confocor 3 FCS system. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. December 2011.

2010:Spectral imaging using Spectral-based Olympus FluoView 1000 CLSM. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. January 2010.

2009:FRAP and FRET imaging and analysis. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. October 2009.

2006-2007: Corso di “Elementi di Bioinformatica” (12 ore totali) tenuto da Dr.Pietro Lio’, University of Cambridge presso Universita’ degli studi di Firenze.

PREMI E RICONOSCIMENTI:

2014: Anton Lang Memorial Award for Research Excellence, MSU-DOE Plant Research Laboratory, Michigan State University (Michigan). The Anton Lang Memorial Fund, established in 1997, supports the annual Anton Lang Memorial Awards and Seminar. Established in memory of the Plant Research Laboratories (PRL) founding director, monies from this fund are used each year to honor one postdoctoral associate who exemplify the research excellence, ideals, dedication, and vision of Anton Lang. Recipients are presented with a memento from the PRL (a handsome walnut clock engraved with their name) and a monetary award, and their names are added to a plaque in the Plant Biology building’s Anton Lang display case.

2013: National Science Foundation competitive travel fellowship (funds covered registration, travel and accommodation costs) to attend the Midwest Plant Cell Dynamics Meeting- Madison, WI-USA, June 5^th to June 7^th. 

2012: National Science Foundation competitive travel fellowship (funds covered registration, travel and accommodation costs) to attend the Midwest Plant Cell Dynamics Meeting- Madison, WI-USA, June 20^th-22^nd.

2008: University of Florence scholarship – Physiological and qualitative parameter studies to build up growth model for Vitis vinifera.

2005-2006: Graduate Teaching Assistantships. The students are required to work as instructor from September to April. University of Saskatchewan-Canada

2004-2006: Graduate Scholarships (UGS).The students receive $16,000 per year subject to maintaining acceptable academic standing. University of Saskatchewan-Canada

ATTIVITA’ EXTRACURRICULARI:

2020 to present: Associate Editor of Frontiers in Plant Metabolism and Chemodiversity;

2019 to present: Editor Board member of Plants (MDPI)

2020 to present: Guest Editor in Cells for the Special Issue “New Insight in Molecular and Cellular Mechanism of Plant Growth”.

2020: Guest Associate Editor in Plant Abiotic Stress-Frontiers in Plant Science. Research Topic:Salt Tolerance: Molecular and Physiological Mechanisms and Breeding Applications

2019: Guest Associate Editor in Plant Abiotic Stress-Frontiers in Plant Science. Research Topic: New Insights into Salinity Sensing, Signaling and Adaptation in Plants.

From 2017: Review Editor in Plant Abiotic Stress (Frontiers in Plant Science).

2016/2018: Seminar Committee (responsabile organizzazione seminari di dipartimento).

2016: Poster Committee (responsabile della selezione di presentazioni e poster durante il rinnovo del grant proposal del Plant Research Laboratory- Department Of Energy).

2015/2016: Personnel Affairs Committee (responsabile dell`orientamento e di fornire informazione riguardanti il Plant Research Laboratory (Michigan State University) ai nuovi studenti in visita, post docs e studenti di dottorato).

2014-present: member of APC (Academic Personnel Council)(consiglio accademico).

2014/2015: Representative Research Associate for APC (rappresentante dei ricercatori incaricato a presenziare e partecipare alle riunioni di facolta`).

ATTIVITA’ DI REVISORE PER RIVISTRE SCIENTIFICHE:

Nature Communications

Frontiers in Plant Science: Plant abiotic stress

New Phytologist

Journal of Experimental Botany

Plant Journal

The Plant Cell

Plant Physiology

Plant and Cell Physiology

Frontiers Plant Science:Plant Metabolism and Chemodiversity

Plant Signaling & Behavior

Plant Physiology and Biochemistry

Planta

Dove Medical Press

COLLABORAZIONI ATTUALI E PASSATE:

Stefano Mancuso, Plant physiology, Department of Plant, Soil and Environmental Science, University of Florence, Italy.

Lucia Gardini, Biophotonics Laboratory, Istituto Nazionale di Ottica” (INO-CNR), Florence, Italy.

Agnieszka Zienkiewicz, Autophagy/lipidomics, Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Poland.

Krzysztof Zienkiewicz, Plant and algal biotechnology and biochemistry, Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Poland.

Sergey Shabala /Plant adaptive responses to environment (salinity, oxidative stress, extreme temperatures, soil acidity, drought, waterlogging, nutritional disorders, biotic stresses)/(University of Tasmania), Australia.

Frantisek Baluska/Cytoskeleton and Vesicular Trafficking/University of Bonn, Germany     .

PUBBLICAZIONI:

1. Zienkiewicz A, Zienkiewicz, Poliner E, Pulman J.A, Du Z, Stefano G, Tsai C, Horn P , Feussner I , Farre E.M , Childs K.L, Brandizzi F , Benning C. The Microalga Nannochloropsis during transition from quiescence to autotrophy in response to nitrogen availability. Plant Physiol. 2020 Feb;182(2):819-839. doi: 10.1104/pp.19.00854.
2. Stefano G and Brandizzi F. Analysis of Endoplasmic Reticulum-Endosomes association using live-cell imaging in plant cells. Methods in Molecular Biology 2020;2177:23-33.
3. Barozzi F, Papadia P, Stefano G, Renna L, Brandizzi F, Migoni D, Fanizzi FP, Piro G, Di Sansebastiano GP. Variation in Membrane Trafficking Linked to SNARE AtSYP51 Interaction With Aquaporin NIP1;1. Front Plant Sci. 2019 Jan 9;9:1949.
4. Renna L, Stefano G, Wormsbaecher C, Slabaugh E, Sulpizio A, Zienkiewicz K, Brandizzi F. (2018) TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network. Nature Commun. 14;9(1):5313.
5. Lai YS, Stefano G, Zemelis-Durfee S, Ruberti C, Gibbons L, Brandizzi F. (2018) Systemic signaling contributes to the unfolded protein response of the plant endoplasmic reticulum. Nature Commun. 25;9(1):3918.
6. Stefano G, Renna L, Wormsbaecher C, Gamble J, Zienkiewicz K, Brandizzi F. (2018) Plant endocytosis requires the ER membrane–anchored proteins VAP27-1 and VAP27-3. Cell Rep. May 22;23(8):2299-2307.
7. Stefano G and Brandizzi F. (2018) Advances in plant ER architecture and dynamics. Plant Physiol. 2018 Jan;176(1):178-186.
8. Stefano G, Renna L, Brandizzi F. (2018) Vacuolar staining and fluorescent probes. Methods Mol Biol, Springer. 1789:55-63.
9. Renna L, Stefano G, Brandizzi F. (2018) Live cell Imaging and Confocal Microscopy. Methods Mol Biol, Springer. 1789:117-130.
10. Cao P, Renna L, Stefano G, Brandizzi F. (2016) SYP73 Anchors the ER to the Actin Cytoskeleton for Maintenance of ER Integrity and Streaming in Arabidopsis. Curr Biol. 5;26(23):3245-3254.
11. Larkin RM, Stefano G, Ruckle ME, Stavoe A, Sinkler CA, Brandizzi F, Malmstrom CM, Osteryoung K. (2016) REDUCED CHLOROPLAST COVERAGE genes from Arabidopsis thaliana establish the size of the chloroplast compartment. PNAS. Feb 23;113(8).
12. Ueda H, Yokota E, Kuwata K, Kutsuna N, Mano S, Shimada T, Tamura K, Stefano G, Fukao Y, Brandizzi F, Shimmen T, Nishimura M, and Hara-Nishimura I (2016). Phosphorylation of the C-terminus of RHD3 Has a Critical Role in the Homotypic ER Membrane Fusion in Arabidopsis. Plant Physiol. Feb;170(2):867-80.
13. Stefano G, Renna L, Lai Y, Slabaugh E, Mannino N, Buono R.A, Otegui M.S, Brandizzi F. (2015) ER network homeostasis is critical for plant endosome streaming and endocytosis. Cell Discovery 1, 15033. doi:10.1038/celldisc.2015.33
14. Stefano G, Renna L, Brandizzi F (2015) BiFC for protein-protein interactions and protein topology: Discussing an integrative approach for an old technique. Methods Mol Biol. 1242:173-82.
15. Ruberti C, Kim SJ, Stefano G, Brandizzi F. (2015) Unfolded protein response in plants: one master, many questions. Curr Opin Plant Biol. Oct; 27:59-66.
16. Wu H, Shabala L, Zhou M, Stefano G, Pandolfi C, Mancuso S, Shabala S. (2015) Developing and validating a high-throughput assay for salinity tissue tolerance in wheat and barley. Planta. Oct; 242(4):847-57.
17. Stefano G, Hawes C, Brandizzi F (2014) ER - the key to the highway. Current Opinion in Plant Biology. Dec; 22:30-8.
18. Stefano G and Brandizzi F (2014) Unique and conserved features of the ER-shaping GTPase RHD3. Cell Logist. 2014 Jan 1;4(1):e28217. 
19. Maneta-Peyret L, Lai YS, Stefano G, Fouillen L, Brandizzi F, Moreau P. (2014) Phospholipid biosynthesis increases in RHD3-defective mutants. Plant Signal Behav. 2014;9(9):e29657. doi: 10.4161/psb.29657
20. Lai Y, Stefano G, and Brandizzi F (2014) ER stress signaling requires RHD3, a functionally conserved ER-shaping GTPase. J Cell Sci. May 29.
21. Stefano G, Renna L, Brandizzi F (2014) The endoplasmic reticulum exerts control over organelle streaming during cell expansion. J Cell Sci. Jan 14.
22. Rodriguez-López J, Martínez-Centeno C, Padmanaban A, Guillén G, Olivares JE, Stefano G, Lledías F, Ramos F, Ghabrial SA, Brandizzi F, Rocha-Sosa M, Díaz-Camino C, Sanchez F (2014) Nodulin 22, a novel small heat-shock protein of the endoplasmic reticulum, is linked to the unfolded protein response in common bean. Mol Plant Microbe Interact. Jan; 27(1): 18-29.
23. Takagi J, Renna L, Takahashi H, Koumoto Y, Tamura K, Stefano G, Fukao Y, Kondo M, Nishimura M, Shimada T, Brandizzi F, Hara-Nishimura I (2013) MAIGO5 functions in protein export from golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell. Nov; 25(11): 4658-75.
24. Mehrshahi P, Stefano G, Andaloro JM, Brandizzi F, Froehlich JE, DellaPenna D (2013) Transorganellar complementation redefines the biochemical continuity of endoplasmic reticulum and chloroplasts. Proc Natl Acad Sci U S A. Jul 16; 110(29): 12126-31. 
25. Stefano G, Osterrieder A, Hawes C., Brandizzi F (2013) Endomembrane and Golgi traffic in plant cells. Methods Cell Biol. 118: 69-83.
26. Renna L, Stefano G, Majeran W, Micalella C, Meinnel T, Giglione C, Brandizzi F (2013) Golgi traffic and integrity depend on N-myristoyl transferase-1 in Arabidopsis. Plant Cell. May; 25(5): 1756-73.
27. Du W, Tamura K, Stefano G, Brandizzi F (2013) The integrity of the plant Golgi apparatus depends on cell growth-controlled activity of GNL1. Mol Plant. May; 6(3): 905-15
28. Tan YJ, Wang WH, Zheng Y, Dong J, Stefano G, Brandizzi F, Garavito RM, Reid GE, Bruening ML (2012) Limited proteolysis via millisecond digestions in protease-modified membranes. Anal Chem. Oct 2; 84(19): 8357-63.
29. Breuers FKH, Braeutigam A, Geimer S, Welzel UY, Stefano G, Renna L, Brandizzi F and Weber APM (2012) Dynamic remodeling of the plastid envelope membranes – a tool for chloroplast envelope in vivo localizations. Front Plant Sci. Jan 23; 3:7.
30. Stefano G, Renna L, Brandizzi F (2012) Fluorescence microscopy screening and next-generation sequencing: useful tools for the identification of genes involved in organelle integrity. J Vis Exp. Apr 13; (62).
31. Stefano G, Renna L, Moss T, McNew JA, Brandizzi F (2012) In Arabidopsis, the spatial and dynamic organization of the endoplasmic reticulum and Golgi apparatus is influenced by the integrity of the C-terminal domain of RHD3, a non-essential GTPase. Plant J. Mar; 69(6): 957-66.
32. Cuin TA, Bose J, Stefano G, Jha D, Tester M, Mancuso S, Shabala S (2011) Assessing the role of root plasma membrane and tonoplast Na(+) /H(+) exchangers in salinity tolerance in wheat: in planta quantification methods. Plant Cell Environ. Jun; 34(6): 947-961.
33. Chen J, Stefano G, Brandizzi F, Zheng H (2011) Arabidopsis RHD3 mediates the generation of the tubular ER network and is required for Golgi distribution and motility in plant cells. J Cell Sci. Jul1; 124(Pt 13): 2241-52.
34. Wegner LH, Stefano G, Shabala L, Rossi M, Mancuso S, Shabala S (2011) Sequential depolarization of root cortical and stelar cells induced by an acute salt shock - implications for Na(+) and K(+) transport into xylem vessels. Plant Cell Environ. May; 34(5): 859-69.
35. *Stefano G, Renna L, Rossi M, Azzarello E, Pollastri S, Brandizzi F, Baluska F and Mancuso S (2010) AGD5 is a GTPase-activating protein at the trans-Golgi network. Plant J. Dec; 64(5): 790-799. (*Corresponding author).
36. Marti L*, Stefano G*, Tamura K, Hawes C, Renna L, Held MA, Brandizzi F (2010) A missense mutation in the vacuolar protein GOLD36 causes organizational defects in the ER and aberrant protein trafficking in the plant secretory pathway. Plant J. Sep; 63(6): 901-913  (*equal contribution).
37. Neumann PM, Weissman R, Stefano G, Mancuso S (2010) Accumulation of xylem transported protein at pit membranes and associated reductions in hydraulic conductance. J Exp Bot. 61(6): 1711-1717.
38. Marti L, Fornaciari S, Renna L, Stefano G, Brandizzi F (2010) COPII-mediated traffic in plants. Trends Plant Sci. Sep; 15(9): 522-528.
39. Agati G, Stefano G, Biricolti S, Tattini M (2009) Mesophyll distribution of 'antioxidant' flavonoid glycosides in Ligustrum vulgare leaves under contrasting sunlight irradiance. Annals of Botany. Oct; 104(5): 853-861.
40. Masi E, Ciszak M, Stefano G, Renna L, Azzarello E, Pandolfi C, Mugnai S, Baluška F, Arecchi FT, Mancuso S (2009) Spatio-temporal dynamics of the electrical network activity in the root apex. PNAS. 106(10): 4048-4053.
41. Masi E, Ciszak M, Mugnai S, Azzarello E, Pandolfi C, Renna L, Stefano G, Voigt B, Volkmann D, Mancuso S (2008) Electrical Network Activity In Plant Roots Under Gravity-Changing Conditions. J of Gravitational Physiology. 15:1.     
42. Mugnai S, Pandolfi C, Azzarello E, Masi E, Renna L, Stefano G, Voigt B, Volkmann D, Mancuso S (2008) Root Apex Physiological Response To Temporary Changes in Gravity Conditions: an Overview on Oxygen AND Nitric Oxide Fluxes. J of Gravitational Physiology 15:1.
43. Matheson LA, Hanton SL, Rossi M, Latijnhouwers M, Stefano G, Renna L, Brandizzi F (2007) Multiple Roles of ARF1 in Plant Cells Include Spatially-Regulated Recruitment of Coatomer and Elements of the Golgi Matrix. Plant Physiol. Apr; 143(4): 1615-1627.
44. Stefano G, Renna L, Chatre L, Hanton SL, Moreau P, Hawes C and Brandizzi F (2006) In tobacco leaf epidermal cells the integrity of protein export from the endoplasmic reticulum and of ER export sites depends on active COPI machinery. Plant Journal. Apr; 46(1): 95-110.
45. Stefano G, Renna L, Chatre L, Hanton SL, Haas TA, Brandizzi F (2006) Arl1 plays a role in the binding of a grip domain of a peripheral matrix protein to the Golgi apparatus in plant cell. Plant Mol Biol. Jun; 61(3): 431-449.
46. Hanton SL, Renna L, Bortolotti LE, Chatre L, Stefano G, Brandizzi F (2005) Diacidic Motifs Influence the Export of Transmembrane Proteins from the Endoplasmic Reticulum in Plant Cells. Plant Cell. Nov; 17(11): 3081-3093.
47. Renna L, Hanton SL, Stefano G, Bortolotti L, Misra V, Brandizzi F (2005) Identification and characterization of AtCASP, a plant transmembrane Golgi matrix protein. Plant Mol Biol. May; 58(1): 109-122.
48. Hanton SL, Bortolotti L, Renna L, Stefano G, Brandizzi F (2005) Crossing the divide--transport between the endoplasmic reticulum and Golgi apparatus in plants. Traffic. Apr; 6(4): 267-277.

Abstracts and Presentations:

2019: Stefano G and Brandizzi F- Novel players in plant clathrin-mediated endocytosis. 22nd Meeting of the European Network for Plant Endomembrane Research- ENPER, September 3-6, 2019. Universidad de Valencia, Spain (speaker).

2019: Stefano G and Brandizzi F- Novel players in plant clathrin-mediated endocytosis. 22nd Penn State Symposium in Plant Biology - Plant Cell Dynamics VIII.June 18 - 21, 2019. University Park, Pennsylvania, USA (Poster presentation).

2017: Stefano G and Brandizzi F- Plant clathrin-mediated endocytosis requires the ER membrane–anchored proteins PVA11 and PVA12 . 53rd annual meeting of the Canadian Botanical Association. July 4-8, 2017. Wilfrid Laurier University, Waterloo, ON, CANADA (invited speaker). 

2013: Stefano G and Brandizzi F-Functional and morphological integrity of the organelles in plant cells. Midwest plant cell dynamics, June 5-7.University of Wisconsin, Madison, USA (speaker).

2012: Stefano G and Brandizzi F-Spatial and dynamic organization of the early secretory organelles in plant cells. Midwest plant cell dynamics, June 20-22. University of Wisconsin, Madison, USA (speaker).

2012: Stefano G and Brandizzi F- Spatial and dynamic organization of the early secretory organelles in plant cells. 15^th European Plant Endomembrane meeting, August 29-31. Madrid, Spain (speaker).

2011: Stefano G and Brandizzi F. -Golgi and endoplasmic reticulum integrity in the plant early secretory pathway. iGRAD-Plant Seminar Series. Heinrich-Heine-Universität Düsseldorf , Germany (invited speaker).

2011: Stefano G and Brandizzi F-Integrity of the early secretory pathway organelles in plant cells. 22^nd International conference on Arabidopsis research, June 22-26. University of Wisconsin, Madison, USA (speaker).

2009: Renna L, Stefano G, Baluska F, Mancuso S- AGD5, an ARF-GAP, interacts with ARF1 GTPase at the Trans-Golgi Network. 5^th International Symposium on Plant Neurobiology, June 25-29. Firenze, Italy.

2009: Pandolfi C, Masi E, Mugnai S, Azzarello E, Renna L, Stefano G, Mancuso S- Physiological response to temporary changes in gravity conditions on plants. In: Physiological response to temporary changes in gravity conditions on plants. ELGRA Symposium, September 1-4. Bonn, Germany.

2008: Masi E, Ciszak M, Pandolfi C, Azzarello E, Mugnai S, Renna L, Stefano G, Voigt B, Mancuso S- Characteristics of the artificial electrical activity generated in plant roots under artificial changes in gravity. 4rd International Symposium on Plant Neurobiology, June 6-9. Fukuoka, Japan.

2008: Mugnai S, Pandolfi C, Azzarello E, Masi E, Renna L, Stefano G, Voigt B, Volkmann D, Mancuso S- Root Apex Physiological Response To Temporary Changes In Gravity Conditions: An Overview On Oxygen And Nitric Oxide Fluxes. In: Life In Space For Life On Earth. June 22-27. Angers, France.

2008: Mancuso S, Masi E, Ciszak M, Mugnai S, Azzarello E, Pandolfi C, Renna L, Stefano G, Voigt B, Volkmann D- Characteristics Of The Electrical Network Activity Generated In Plant Roots Under Changes In Artificial Gravity. In: 4th Symposium On Plant Neurobiology. June 4-7. Fukuoka, Japan.

2008: Mancuso S, Masi E, Ciszak M, Stefano G, Renna L, Azzarello E, Pandolfi C, Mugnai S, Baluska F, Arecchi T- Neuroid Conduction In Plants. In: 4th Symposium On Plant Neurobiology. June 4-7. Fukuoka, Japan.

2008: Masi E, Mugnai S, Azzarello E, Ciszak M, Pandolfi C, Renna L, Stefano G, Voigt B, Volkmann D, Mancuso S- Electrical network activity in plant roots under gravity-changing conditions. In: Symposium "Life in Space for Life on Earth." June 22-27. Angers, France.

2007: Masi E, Ciszak M, Montina A, Malachovska V, Mugnai S, Azzarello E, Pandolfi C, Renna L, Stefano G, Voigt B, Hlavacka A, Arecchi FT, Mancuso S- Spatio-temporal dynamics of the electrical network activity in the root apex. A multi-electrode array (MEA) study. 3rd International Symposium on Plant Neurobiology. May 14-18. Štrbské pleso, Slovakia.

2006: Stefano G and Brandizzi F. -Trans Golgi Network dynamics: a new light in plants.Department of Experimental Plant Biology. Prague, Czech Republic (invited speaker).

2006: Matheson L, Stefano G, Brandizzi F- Matrix protein targeting to the plant Golgi apparatus. Plant Biology: August 5-9. Boston, Massachusetts, USA.

2006: Matheson L, Stefano G, Brandizzi F- Dissection of the molecular mechanisms for matrix protein targeting to the plant Golgi apparatus. 23rd Annual Meeting of the International Society for Analytical Cytology", May 20-24. Quebec City, Quebec, Canada.

2004: Brandizzi F, Hawes C, Hanton SL, Renna L, Stefano G- Use of fluorescent proteins to dissect the dynamics of the plant secretory pathway. European Microscopy Congress, Antwerpen, Belgium.

Competenze informatiche

Software: R, Matlab, Origin 8, Graphpad Prism, Unigene, Microsoft Office (Excel, Power Point, Word, Onenote), Photoshop, Illustrator, Inkscape, GIMP, Fiji/ImageJ.

Operating Systems: Mac OS, Linux & Windows.

Competenze tecniche

1) Advanced Confocal Microscopy:

- FRAP (Fluorescence recovery after photobleaching), iFRAP (inverse FRAP), 2-color FRAP, FRET (fluorescence resonance energy transfer)

-Total Internal Reflection Fluorescence (TIRF) variant, also named Variable Epifluorescence Microscope VAEM)

- Stochastic Optical Reconstruction Microscopy (STORM)

-Multi-channnel fluorescence imaging,

-High-speed resonance scanning

-3D rendering and animation

-Co-localization analysis

-Cell counting and image analysis software

-Live cell imaging and quantitative analysis over time

-FRAP and FRET imaging and analysis

-BiFC analysis

-Spectral imaging

-Fluorescence Correlation Spectroscopy (FCS)

2) TEM ( transmission electron microscope) and Ultramicrotome (Leica)

3) Uso di ULTRA Microplate fluorescence Reader per misurare Fluorescence intensity, BRET, FRET, and Luminescence.

4) qPCR; quantitative gene expression analysis using real-time qPCR.

5)Yeast Two-Hybrid (Y2H) Screening

6)Biochemical/Molecular biology techniques used: SDS-PAGE, Agarose Gel

Electrophoresis, Co-immunoprecipitation, protein expression and purification via affinity

chromatography (Ni-NTA, Cobalt, glutathione columns), protein assays, PCR, RT-PCR, western blot, silver stains, enzyme kinetics, protein-protein interaction technique; protein pull down assays.

7) Conoscenze pratiche e teoriche di tecniche di biologia cellulare e biochimica: isolamento di organelli, estrazione di proteine mediante SDS PAGE; cromatografia su carta, su colonna e su strato sottile; elettroforesi ad alto voltaggio su carta.

8) Esperienza nelle metodologie di crescita in ambiente controllato di specie vegetali: Nicotiana tabacum ed Arabidopsis thaliana; esperienza nelle metodologie di allestimento e mantenimento di colture cellulari vegetali in terreni liquidi e solidi. Colture di lieviti.

Affiliation

(University of Florence)

         Department of Biology

         Via Micheli 3, 

       Firenze, 50121, Italy

tel. office +39 055 4574065

https://www.unifi.it/p-doc2-2020-0-A-2c303b2e3429-1.html  ;

https://sites.google.com/unifi.it/giovannistefano/home-page

WORK EXPERIENCE

09/2020 – present

Assistant Professor (RTDA-Fixed Term)

Faculty of Mathematics and Natural Sciences/ Department of Biology, University of Florence/ Italy

▪              Teaching and research activities

Plant Cell and Molecular Biology

01/2020 –08/2020

Assistant Professor (Fixed Term)

Faculty of Mathematics and Natural Sciences/ Department of Biology, Michigan State University, / USA

▪          Research activities

Plant Cell and Molecular Biology

09/2014 –12/2019

Assistant Professor (Fixed Term)

Faculty of Mathematics and Natural Sciences/ Department of Energy (DOE) Plant Research Laboratory, Michigan State University, / USA

▪              Research activities and Research supervisor

Plant Cell and Molecular Biology

09/2009 –08/2014

Post-doctoral research associate

Faculty of Mathematics and Natural Sciences/ Department of Energy (DOE) Plant Research Laboratory, Michigan State University, / USA

▪              Research activities and Research supervisor

Plant Cell and Molecular Biology

EDUCATION AND TRAINING

11/2018

Full Professor National Scientific Habilitationin Botany 05/A1

MIUR (Ministero dell'Università e della Ricerca, Italy)

▪              Botany 05/A1

01/2020

Associate Professor National Scientific Habilitationin Molecular Biology 05/E2

MIUR (Ministero dell'Università e della Ricerca, Italy)

▪              Molecular Biology 05/E2

09/2017-11/2017

Teaching certificate - “Pathways to Scientific Teaching”

Faculty of Mathematics and Natural Sciences/ Department of Biology, Michigan State University,USA

▪              Plant Biology

03/2006-08/2009

Doctor of Philosophy (Ph.D.)

Faculty of Mathematics and Natural Sciences/Department of Biology, University of Bonn (GERMANY)

▪              Plant Cell and Molecular Biology

01/2004-01/2006

Master of Science (M.Sc.)

Faculty of Mathematics and Natural Sciences/Department of Biology, University of Saskatchewan (CANADA)

▪              Plant Cell and Molecular Biology

2003

Licence ofProfessional Biologist

MIUR (Ministero dell'Università e della Ricerca, Italy)

▪              Biology

1996-2002

Degree in Biological Sciences

Faculty of Mathematics and Natural Sciences/Department of Biology, Universita’ del Salento (Italy).

▪              Biology

2019

Use of the transmission electron microscope (TEM) Jeol 1400 flash. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. November 2019.

2019

Light sheet microscopy. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. October 2019.

2019

Workshop PhotosynQ Hands-on Lead by Prof. David Kramer): how to operate and use the MultispeQ: 1. Overview of Applications and MultispeQ Fundamentals 2. Learn to navigate the apps and operate your instrument. 3. Project Generation and Experiment Design. 4. Live demonstration creating a project using existing protocols and best practices for experiment efficiencies and accuracy. 5. Hands on Data Collection 6. Actual field data collection in small teams. 7 Evaluation of data. 8.Live demonstration of analyses of real data sets using online PhotosynQ tools. April 2019.

2018

Strategies for teaching science practices using free technologies at MSU (Michigan State University), 13 Nov 2018.

2017

Write Winning Grant Proposals. John D. Robertson, Grant Writers' Seminars and Workshops, LLC. Presentor: John D. Robertson, Ph.D., associate member of the Grant Writers' Seminars and Workshops, LLC. Idea development, Identification of the most appropriate granting agency, How to write for reviewers, Tips and strategies that are of proven value in presenting an applicant's case to reviewers. January 2017.

2016

“Pathways to Scientific Teaching” (by Prof. Diane Ebert-May, Michigan State University) September-November 2016.

2016

Use of the transmission electron microscope (TEM) and preparative equipment in the biological sciences. Sectioning for electron microscopy. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. December 2016.

2015

Total Internal Reflection Fluorescence (TIRF) microscopy and Super Resolution STORM imaging usingNikon A1 CLSM. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. June 2015.

2014

Laser Capture Microscopy (LCM) using Zeiss Palm MicroBeam IV Laser Capture Microscope. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. February 2014.

2013

Workshop: Fluctuation cross-correlation analysis for the study of fluorescent reporters in tip growth (organized by Luis Vidali) University of Wisconsin, Madison, USA. June 2013.

2013

Workshop: Kinematics (organized by Wendy Silk, Edgar Spalding, and Nathan Miller) University of Wisconsin, Madison, USA. June 2013.

2012

Workshop: Image signal quantification (Kevin Eliceiri) University of Wisconsin, Madison, USA. June 2012.

2012

Workshop: Implementation of computational models: entry points and first steps (Anja Geitmann) University of Wisconsin, Madison, USA. June 2012.

2011

Fluorescence Correlation Spectroscopy using Zeiss LSM Confocor 3 FCS system. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. December 2011.

2010

Spectral imaging using Spectral-based Olympus FluoView 1000 CLSM. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. January 2010.

2009

FRAP and FRET imaging and analysis. Center for Advanced Microscopy, Michigan State University Core Facility, East Lansing-MI, USA. October 2009.

2006-2007

Course of "Elements of Bioinformatics" (12 hours total) held by Dr. Pietro Lio', University of Cambridge at the University of Florence.

PERSONAL SKILLS

Mother tongue(s)

Italian

Other language(s)

English

French

Job-related skills

expert in the following techniques:

1) Advanced Confocal Microscopy:

- FRAP (Fluorescence recovery after photobleaching), iFRAP (inverse FRAP), 2-color FRAP, FRET (fluorescence resonance energy transfer)

-Total Internal Reflection Fluorescence (TIRF) variant, also named Variable Epifluorescence Microscope VAEM)

- Stochastic Optical Reconstruction Microscopy (STORM)

-Multi-channnel fluorescence imaging,

-High-speed resonance scanning

-3D rendering and animation

-Co-localization analysis

-Cell counting and image analysis software

-Live cell imaging and quantitative analysis over time

-FRAP and FRET imaging and analysis

-BiFC analysis

-Spectral imaging

-Fluorescence Correlation Spectroscopy (FCS)

2) TEM ( transmission electron microscope) and Ultramicrotome (Leica)

3) Use of the ULTRA Microplate Fluorescence Reader for measuring Fluorescence intensity, BRET, FRET, and Luminescence.

4) qPCR; quantitative gene expression analysis using real-time qPCR.

5) Experience with yeast cultures; Yeast Two-Hybrid (Y2H) Screening.

6) Biochemical/Molecular biology techniques used: SDS-PAGE, Agarose Gel

Electrophoresis, Co-immunoprecipitation, protein expression and purification via affinity

chromatography (Ni-NTA, Cobalt, glutathione columns), protein assays, PCR, RT-PCR, western blot, silver stains, enzyme kinetics, protein-protein interaction technique; protein pull down assays.

7) Practical and theoretical knowledge of cellular biology and biochemistry techniques: organelle isolation, protein extraction using SDS-PAGE; paper chromatography, column chromatography, and thin-layer chromatography; high-voltage electrophoresis on paper.

8) Experience in controlled environment growth methodologies of plant species: Nicotiana tabacum and Arabidopsis thaliana; proficiency in establishing and maintaining plant cell cultures in both liquid and solid mediums.

Digital skills

Software: R, Matlab, Origin 8, Graphpad Prism, Unigene, Microsoft Office (Excel, Power Point, Word, Onenote), Photoshop, Illustrator, Inkscape, GIMP, Fiji/ImageJ.

Operating Systems: Mac OS, Linux & Windows.

WORK ACTIVITIES

Awards

2014: Anton Lang Memorial Award for Research Excellence, MSU-DOE Plant Research Laboratory, Michigan State University (Michigan)/USA.

2013: National Science Foundation competitive travel fellowship/ University of Madison (Wisconsin)/USA

2012: National Science Foundation competitive travel fellowship/ University of Madison (Wisconsin)/USA

2008: University of Florence scholarship – Physiological and qualitative parameter studies to build up growth model for Vitis vinifera. Faculty of Agriculture/ Department of Plant,

Soil and Environmental Science, University of Florence/ ITALY

2005 – 2006: Graduate Teaching Assistantships/ Faculty of Mathematics and Natural Sciences/ Department of Biology, University of Saskatchewan/ CANADA

2004 – 2006: Graduate Scholarships (UGS)/ Faculty of Mathematics and Natural Sciences/ Department of Biology, University of Saskatchewan/ CANADA

Editorial activity

2020-present: Frontiers inPlant Metabolism and Chemodiversity (Associate Editor);

2019-present: Plants (Editorial Board Member);

2020: Frontiers in Plant Science; Guest Editor in Cells for the Special Issue “New Insight in Molecular and Cellular Mechanism of Plant Growth”.

2020: Frontiers in Plant Science; Guest Associate Editor in Plant Abiotic Stress-Frontiers in Plant Science. Research Topic:Salt Tolerance: Molecular and Physiological Mechanisms and Breeding Applications

2019: Frontiers in Plant Science; Guest Associate Editor in Plant Abiotic Stress-Frontiers in Plant Science. Research Topic: New Insights into Salinity Sensing, Signaling and Adaptation in Plants.

2022: Cells(guest editor).

Review Editor in Nature Communications; Frontiers in Plant Science: Plant abiotic stress; New Phytologist; Journal of Experimental Botany; Plant Journal; The Plant Cell; Plant Physiology; Plant and Cell Physiology; Frontiers Plant Science: Plant Metabolism and Chemodiversity; Plant Signaling & Behavior; Plant Physiology and Biochemistry; Planta

Abstract and presentations

2019: Stefano G. and Brandizzi F- Novel players in plant clathrin-mediated endocytosis. 22^nd Meeting of the European Network for Plant Endomembrane Research- ENPER, September 3-6, 2019. Universidad de Valencia, Spain (speaker).

2019: Stefano G. and Brandizzi F- Novel players in plant clathrin-mediated endocytosis. 22^nd Penn State Symposium in Plant Biology - Plant Cell Dynamics VIII.June 18 - 21, 2019. University Park, Pennsylvania, USA (Poster presentation).

2017: Stefano G. and Brandizzi F: Plant clathrin-mediated endocytosis requires the ER membrane–anchored proteins PVA11 and PVA12 . 53rd annual meeting of the Canadian Botanical Association. July 4-8, 2017. Wilfrid Laurier University, Waterloo, ON, CANADA (invited speaker). 

2013: Stefano G and Brandizzi F:Functional and morphological integrity of the organelles in plant cells. Midwest plant cell dynamics, June 5-7.University of Wisconsin, Madison, USA.

2012: Stefano G and Brandizzi F:Spatial and dynamic organization of the early secretory organelles in plant cells. Midwest plant cell dynamics, June 20-22. University of Wisconsin, Madison, USA.

2012: Stefano G and Brandizzi F: Spatial and dynamic organization of the early secretory organelles in plant cells. 15^th European Plant Endomembrane meeting, August 29-31. Madrid, Spain.

2011: Stefano G and Brandizzi F:Integrity of the early secretory pathway organelles in plant cells. 22^nd International conference on Arabidopsis research, June 22-26. University of Wisconsin, Madison, USA.

2009: Renna L, Stefano G, Baluska F, Mancuso S: AGD5, an ARF-GAP, interacts with ARF1 GTPase at the Trans-Golgi Network. 5^th International Symposium on Plant Neurobiology, June 25-29. Firenze, Italy.

2009: Pandolfi C, Masi E, Mugnai S, Azzarello E, Renna L, Stefano G, Mancuso S: Physiological response to temporary changes in gravity conditions on plants. In: Physiological response to temporary changes in gravity conditions on plants. ELGRA Symposium, September 1-4. Bonn, Germany.

2008: Masi E, Ciszak M, Pandolfi C, Azzarello E, Mugnai S, Renna L, Stefano G, Voigt B, Mancuso S: Characteristics of the artificial electrical activity generated in plant roots under artificial changes in gravity. 4rd International Symposium on Plant Neurobiology, June 6-9. Fukuoka, Japan.

2008: Mugnai S, Pandolfi C, Azzarello E, Masi E, Renna L, Stefano G, Voigt B, Volkmann D, Mancuso S: Root Apex Physiological Response To Temporary Changes In Gravity Conditions: An Overview On Oxygen And Nitric Oxide Fluxes. In: Life In Space For Life On Earth. June 22-27. Angers, France.

2008: Mancuso S, Masi E, Ciszak M, Mugnai S, Azzarello E, Pandolfi C, Renna L, Stefano G, Voigt B, Volkmann D: Characteristics Of The Electrical Network Activity Generated In Plant Roots Under Changes In Artificial Gravity. In: 4th Symposium On Plant Neurobiology. June 4-7. Fukuoka, Japan.

2008: Mancuso S, Masi E, Ciszak M, Stefano G, Renna L, Azzarello E, Pandolfi C, Mugnai S, Baluska F, Arecchi T: Neuroid Conduction In Plants. In: 4th Symposium On Plant Neurobiology. June 4-7. Fukuoka, Japan.

2008: Masi E, Mugnai S, Azzarello E, Ciszak M, Pandolfi C, Renna L, Stefano G, Voigt B, Volkmann D, Mancuso S: Electrical network activity in plant roots under gravity-changing conditions. In: Symposium "Life in Space for Life on Earth." June 22-27. Angers, France.

2007: Masi E, Ciszak M, Montina A, Malachovska V, Mugnai S, Azzarello E, Pandolfi C, Renna L, Stefano G, Voigt B, Hlavacka A, Arecchi FT, Mancuso S: Spatio-temporal dynamics of the electrical network activity in the root apex. A multi-electrode array (MEA) study. 3rd International Symposium on Plant Neurobiology.May 14-18. Štrbské pleso, Slovakia.

2006: Matheson L, Stefano G, Brandizzi F: Matrix protein targeting to the plant Golgi apparatus. Plant Biology: August 5-9. Boston, Massachusetts, USA.

2006: Matheson L, Stefano G, Brandizzi F: Dissection of the molecular mechanisms for matrix protein targeting to the plant Golgi apparatus. 23rd Annual Meeting of the International Society for Analytical Cytology", May 20-24. Quebec City, Quebec, Canada.

2004: Brandizzi F, Hawes C, Hanton SL, Renna L, Stefano G: Use of fluorescent proteins to dissect the dynamics of the plant secretory pathway. European Microscopy Congress, Antwerpen, Belgium.

2006: Stefano G and Brandizzi F. Trans Golgi Network dynamics: a new light in plants.Department of Experimental Plant Biology. Prague, Czech Republic (invited speaker).

2009: Stefano G and Brandizzi F. Golgi and endoplasmic reticulum integrity in the plant early secretory pathway. iGRAD-Plant Seminar Series. Heinrich-Heine-Universität Düsseldorf , Germany (invited speaker).

2008                 Scientific support for the Space Biology area at Department of Plant, Soil and Environmental Science, University of Florence (Italy).

2008                 ESA (European Space Agency) Project: 49th Parabolic Flights - Electrical network activity, ROS production and endocytosis dynamics in plant roots under gravity-changing conditions.

2007                 ESA Project: 47th Parabolic Flights - Oxidative burst: Earliest response of roots to changes of gravitational force.

2006                 ESA Project: 46th Parabolic Flights - Electrical network activity and endocytosis dynamics in plant roots under gravity-changing condition.

2020 – present Botany–Faculty of Mathematics and Natural Sciences/ Department of Biology, University of Florence/ Italy

2020 – present Biology and Ecology of Algae–Faculty of Mathematics and Natural Sciences/ Department of Biology, University of Florence/ Italy

2017                 “Pathways to Scientific Teaching”, Faculty of Mathematics and Natural Sciences/ Plant Biology, Michigan State University, / USA

2015                MMG 409 (2 hours)–(Undergraduate Level)– Faculty of Mathematics and Natural Sciences/ MSU-DOE Plant Research Laboratory, Michigan State University, / USA

2011                PLB 800 (2 hours)–Seminar in plant Biology (Graduate Level)– Faculty of Mathematics and Natural Sciences/ MSU-DOE Plant Research Laboratory, Michigan State University/ USA

2008                Graduate level course on Innovative Technologies (2 CFU), confocal microscopy– Faculty of Agriculture/ Department of Plant, Soil and Environmental Science, University of Florence/ Italy

ADDITIONAL INFORMATION

Publications

Publications:

1. Taiti C, Stefano G, Percaccio E, Di Giacomo S, Iannone M, Marianelli A, Di Sotto A, Garzoli S. Addition of Spirulina to Craft Beer: Evaluation of the Effects on Volatile Flavor Profile and Cytoprotective Properties. Antioxidants, 2023 Apr 28;12 (5 :1021. doi: 10.3390/antiox12051021.
2. Wu H, Hill CB, Stefano G, Bose J. New insights into salinity sensing, signaling and adaptation in plants. Frontiers in Plant Science 2021. 11, 1843.
3. Zienkiewicz A, Zienkiewicz, Poliner E, Pulman J.A, Du Z, Stefano G, Tsai C, Horn P , Feussner I , Farre E.M , Childs K.L, Brandizzi F , Benning C. The Microalga Nannochloropsis during transition from quiescence to autotrophy in response to nitrogen availability. Plant Physiol. 2020 Feb;182(2):819-839. doi: 10.1104/pp.19.00854.
4. Stefano G and Brandizzi F. Analysis of Endoplasmic Reticulum-Endosomes association using live-cell imaging in plant cells. Methods in Molecular Biology 2020;2177:23-33.
5. Barozzi F, Papadia P, Stefano G, Renna L, Brandizzi F, Migoni D, Fanizzi FP, Piro G, Di Sansebastiano GP. Variation in Membrane Trafficking Linked to SNARE AtSYP51 Interaction With Aquaporin NIP1;1. Front Plant Sci. 2019 Jan 9;9:1949.
6. Renna L, Stefano G, Wormsbaecher C, Slabaugh E, Sulpizio A, Zienkiewicz K, Brandizzi F. (2018) TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network. Nature Commun. 14;9(1):5313.
7. Lai YS, Stefano G, Zemelis-Durfee S, Ruberti C, Gibbons L, Brandizzi F. (2018) Systemic signaling contributes to the unfolded protein response of the plant endoplasmic reticulum. Nature Commun. 25;9(1):3918.
8. Stefano G, Renna L, Wormsbaecher C, Gamble J, Zienkiewicz K, Brandizzi F. (2018) Plant endocytosis requires the ER membrane–anchored proteins VAP27-1 and VAP27-3. Cell Rep. May 22;23(8):2299-2307.
9. Stefano G and Brandizzi F. (2018) Advances in plant ER architecture and dynamics. Plant Physiol. 2018 Jan;176(1):178-186.
10. Stefano G, Renna L, Brandizzi F. (2018) Vacuolar staining and fluorescent probes. Methods Mol Biol, Springer. 1789:55-63.
11. Renna L, Stefano G, Brandizzi F. (2018) Live cell Imaging and Confocal Microscopy. Methods Mol Biol, Springer. 1789:117-130.
12. Cao P, Renna L, Stefano G, Brandizzi F. (2016) SYP73 Anchors the ER to the Actin Cytoskeleton for Maintenance of ER Integrity and Streaming in Arabidopsis. Curr Biol. 5;26(23):3245-3254.
13. Larkin RM, Stefano G, Ruckle ME, Stavoe A, Sinkler CA, Brandizzi F, Malmstrom CM, Osteryoung K. (2016) REDUCED CHLOROPLAST COVERAGE genes from Arabidopsis thaliana establish the size of the chloroplast compartment. PNAS. Feb 23;113(8).
14. Ueda H, Yokota E, Kuwata K, Kutsuna N, Mano S, Shimada T, Tamura K, Stefano G, Fukao Y, Brandizzi F, Shimmen T, Nishimura M, and Hara-Nishimura I (2016). Phosphorylation of the C-terminus of RHD3 Has a Critical Role in the Homotypic ER Membrane Fusion in Arabidopsis. Plant Physiol. Feb;170(2):867-80.
15. Stefano G, Renna L, Lai Y, Slabaugh E, Mannino N, Buono R.A, Otegui M.S, Brandizzi F. (2015) ER network homeostasis is critical for plant endosome streaming and endocytosis. Cell Discovery 1, 15033. doi:10.1038/celldisc.2015.33
16. Stefano G, Renna L, Brandizzi F (2015) BiFC for protein-protein interactions and protein topology: Discussing an integrative approach for an old technique. Methods Mol Biol. 1242:173-82.
17. Ruberti C, Kim SJ, Stefano G, Brandizzi F. (2015) Unfolded protein response in plants: one master, many questions. Curr Opin Plant Biol. Oct; 27:59-66.
18. Wu H, Shabala L, Zhou M, Stefano G, Pandolfi C, Mancuso S, Shabala S. (2015) Developing and validating a high-throughput assay for salinity tissue tolerance in wheat and barley. Planta. Oct; 242(4):847-57.
19. Stefano G, Hawes C, Brandizzi F (2014) ER - the key to the highway. Current Opinion in Plant Biology. Dec; 22:30-8.
20. Stefano G and Brandizzi F (2014) Unique and conserved features of the ER-shaping GTPase RHD3. Cell Logist. 2014 Jan 1;4(1):e28217. 
21. Maneta-Peyret L, Lai YS, Stefano G, Fouillen L, Brandizzi F, Moreau P. (2014) Phospholipid biosynthesis increases in RHD3-defective mutants. Plant Signal Behav. 2014;9(9):e29657. doi: 10.4161/psb.29657
22. Lai Y, Stefano G, and Brandizzi F (2014) ER stress signaling requires RHD3, a functionally conserved ER-shaping GTPase. J Cell Sci. May 29.
23. Stefano G, Renna L, Brandizzi F (2014) The endoplasmic reticulum exerts control over organelle streaming during cell expansion. J Cell Sci. Jan 14.
24. Rodriguez-López J, Martínez-Centeno C, Padmanaban A, Guillén G, Olivares JE, Stefano G, Lledías F, Ramos F, Ghabrial SA, Brandizzi F, Rocha-Sosa M, Díaz-Camino C, Sanchez F (2014) Nodulin 22, a novel small heat-shock protein of the endoplasmic reticulum, is linked to the unfolded protein response in common bean. Mol Plant Microbe Interact. Jan; 27(1): 18-29.
25. Takagi J, Renna L, Takahashi H, Koumoto Y, Tamura K, Stefano G, Fukao Y, Kondo M, Nishimura M, Shimada T, Brandizzi F, Hara-Nishimura I (2013) MAIGO5 functions in protein export from golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell. Nov; 25(11): 4658-75.
26. Mehrshahi P, Stefano G, Andaloro JM, Brandizzi F, Froehlich JE, DellaPenna D (2013) Transorganellar complementation redefines the biochemical continuity of endoplasmic reticulum and chloroplasts. Proc Natl Acad Sci U S A. Jul 16; 110(29): 12126-31. 
27. Stefano G, Osterrieder A, Hawes C., Brandizzi F (2013) Endomembrane and Golgi traffic in plant cells. Methods Cell Biol. 118: 69-83.
28. Renna L, Stefano G, Majeran W, Micalella C, Meinnel T, Giglione C, Brandizzi F (2013) Golgi traffic and integrity depend on N-myristoyl transferase-1 in Arabidopsis. Plant Cell. May; 25(5): 1756-73.
29. Du W, Tamura K, Stefano G, Brandizzi F (2013) The integrity of the plant Golgi apparatus depends on cell growth-controlled activity of GNL1. Mol Plant. May; 6(3): 905-15
30. Tan YJ, Wang WH, Zheng Y, Dong J, Stefano G, Brandizzi F, Garavito RM, Reid GE, Bruening ML (2012) Limited proteolysis via millisecond digestions in protease-modified membranes. Anal Chem. Oct 2; 84(19): 8357-63.
31. Breuers FKH, Braeutigam A, Geimer S, Welzel UY, Stefano G, Renna L, Brandizzi F and Weber APM (2012) Dynamic remodeling of the plastid envelope membranes – a tool for chloroplast envelope in vivo localizations. Front Plant Sci. Jan 23; 3:7.
32. Stefano G, Renna L, Brandizzi F (2012) Fluorescence microscopy screening and next-generation sequencing: useful tools for the identification of genes involved in organelle integrity. J Vis Exp. Apr 13; (62).
33. Stefano G, Renna L, Moss T, McNew JA, Brandizzi F (2012) In Arabidopsis, the spatial and dynamic organization of the endoplasmic reticulum and Golgi apparatus is influenced by the integrity of the C-terminal domain of RHD3, a non-essential GTPase. Plant J. Mar; 69(6): 957-66.
34. Cuin TA, Bose J, Stefano G, Jha D, Tester M, Mancuso S, Shabala S (2011) Assessing the role of root plasma membrane and tonoplast Na(+) /H(+) exchangers in salinity tolerance in wheat: in planta quantification methods. Plant Cell Environ. Jun; 34(6): 947-961.
35. Chen J, Stefano G, Brandizzi F, Zheng H (2011) Arabidopsis RHD3 mediates the generation of the tubular ER network and is required for Golgi distribution and motility in plant cells. J Cell Sci. Jul1; 124(Pt 13): 2241-52.
36. Wegner LH, Stefano G, Shabala L, Rossi M, Mancuso S, Shabala S (2011) Sequential depolarization of root cortical and stelar cells induced by an acute salt shock - implications for Na(+) and K(+) transport into xylem vessels. Plant Cell Environ. May; 34(5): 859-69.
37. *Stefano G, Renna L, Rossi M, Azzarello E, Pollastri S, Brandizzi F, Baluska F and Mancuso S (2010) AGD5 is a GTPase-activating protein at the trans-Golgi network. Plant J. Dec; 64(5): 790-799. (*Corresponding author).
38. Marti L*, Stefano G*, Tamura K, Hawes C, Renna L, Held MA, Brandizzi F (2010) A missense mutation in the vacuolar protein GOLD36 causes organizational defects in the ER and aberrant protein trafficking in the plant secretory pathway. Plant J. Sep; 63(6): 901-913  (*equal contribution).
39. Neumann PM, Weissman R, Stefano G, Mancuso S (2010) Accumulation of xylem transported protein at pit membranes and associated reductions in hydraulic conductance. J Exp Bot. 61(6): 1711-1717.
40. Marti L, Fornaciari S, Renna L, Stefano G, Brandizzi F (2010) COPII-mediated traffic in plants. Trends Plant Sci. Sep; 15(9): 522-528.
41. Agati G, Stefano G, Biricolti S, Tattini M (2009) Mesophyll distribution of 'antioxidant' flavonoid glycosides in Ligustrum vulgare leaves under contrasting sunlight irradiance. Annals of Botany. Oct; 104(5): 853-861.
42. Masi E, Ciszak M, Stefano G, Renna L, Azzarello E, Pandolfi C, Mugnai S, Baluška F, Arecchi FT, Mancuso S (2009) Spatio-temporal dynamics of the electrical network activity in the root apex. PNAS. 106(10): 4048-4053.
43. Masi E, Ciszak M, Mugnai S, Azzarello E, Pandolfi C, Renna L, Stefano G, Voigt B, Volkmann D, Mancuso S (2008) Electrical Network Activity In Plant Roots Under Gravity-Changing Conditions. J of Gravitational Physiology. 15:1.     
44. Mugnai S, Pandolfi C, Azzarello E, Masi E, Renna L, Stefano G, Voigt B, Volkmann D, Mancuso S (2008) Root Apex Physiological Response To Temporary Changes in Gravity Conditions: an Overview on Oxygen AND Nitric Oxide Fluxes. J of Gravitational Physiology 15:1.
45. Matheson LA, Hanton SL, Rossi M, Latijnhouwers M, Stefano G, Renna L, Brandizzi F (2007) Multiple Roles of ARF1 in Plant Cells Include Spatially-Regulated Recruitment of Coatomer and Elements of the Golgi Matrix. Plant Physiol. Apr; 143(4): 1615-1627.
46. Stefano G, Renna L, Chatre L, Hanton SL, Moreau P, Hawes C and Brandizzi F (2006) In tobacco leaf epidermal cells the integrity of protein export from the endoplasmic reticulum and of ER export sites depends on active COPI machinery. Plant Journal. Apr; 46(1): 95-110.
47. Stefano G, Renna L, Chatre L, Hanton SL, Haas TA, Brandizzi F (2006) Arl1 plays a role in the binding of a grip domain of a peripheral matrix protein to the Golgi apparatus in plant cell. Plant Mol Biol. Jun; 61(3): 431-449.
48. Hanton SL, Renna L, Bortolotti LE, Chatre L, Stefano G, Brandizzi F (2005) Diacidic Motifs Influence the Export of Transmembrane Proteins from the Endoplasmic Reticulum in Plant Cells. Plant Cell. Nov; 17(11): 3081-3093.
49. Renna L, Hanton SL, Stefano G, Bortolotti L, Misra V, Brandizzi F (2005) Identification and characterization of AtCASP, a plant transmembrane Golgi matrix protein. Plant Mol Biol. May; 58(1): 109-122.
50. Hanton SL, Bortolotti L, Renna L, Stefano G, Brandizzi F (2005) Crossing the divide--transport between the endoplasmic reticulum and Golgi apparatus in plants. Traffic. Apr; 6(4): 267-277.