Drugs active on receptor-channels, on G-protein receptors, on tyrosine kinase-associated receptors, ion channels, enzymes, and carriers. Cellular mechanisms of tolerance and drug dependence. Cytokines: receptors and functions. Active drugs on intracellular receptors and regulation of gene expression. Principles of gene therapy. Stem cells and principles of regenerative medicine. Monoclonal antibodies. Mechanism of action of the main anticancer drugs.
Farmacologia generale e molecolare, di Francesco Clementi, Guido Fumagalli, IV Edizione, Utet EdS.
Knowledge on cellular and molecular mechanisms of drug action; on the drug seen not only as a therapeutic tool, but also as a mean of investigation to study several biological phenomena and, ultimately, to design new and more modern drugs. Cellular and molecular bases for the evaluation of the adverse and toxic effects of drugs and the new frontiers of regenerative medicine.
Skills: understanding of the mechanisms of action of drugs that emerge by the knowledge of molecular biology.
Skills acquired at the end of the course: Fundamental elements for the study of cellular and molecular mechanisms of action of drugs and of practice in a pharmacology laboratory.
Frontal lessons supported by slides, available to students to the UNIFI e-learning platform.
Type of Assessment
Nature and structure of the molecules present in the organism by which the drugs interact and molecular events that underlie the cellular effects of the drugs.
The channel receptors: molecular organization and modulation of the activity of the channel receptors. Cholinergic nicotinic receptor: molecular structure and active drugs on this receptor.
GABAA receptor: structure and agonist and antagonist drugs on this receptor. Benzodiazepines and barbiturates. Glutamate and serotonin channel receptors: molecular structure, function and active drugs on these receptors.
G protein coupled receptors: Molecular organization, function and effector systems.
Growth factor receptors: molecular organization and pharmacological approaches.
Modulation of receptor responses: desensitization and up-regulation.
Target ion channels of drug action: Molecular organization, classification and pharmacology. Antiepileptic and neuroprotective drugs.
Neurotransporters: Molecular organization and active drugs on such targets. Antidepressants and drugs of abuse.
The pumps. Sodium / potassium pump and cardioactive glycoside drugs. Gastric proton pump and anti-ulcer drugs.
The cascade of arachidonic acid. Non-steroidal anti-inflammatory drugs.
Intracellular receptors: Molecular organization and pharmacology modulation. Receptors for glucocorticoids and mineralocorticoids.
Soluble receptors and monoclonal antibodies.
Regenerative medicine and gene therapy. Definitions and main types of stem cells. Applications of cell therapy and regenerative medicine. Purposes, perspectives, technologies for gene transfection.
Cellular and molecular mechanisms of some doping methods.
Mechanism of action at the cellular level of anticancer drugs.
Active drugs on cognitive processes.
Methods for the study of neuroprotective and active drugs on cognitive processes: two experimental models in vitro.
Patch clamp recording from isolated cells: study of the effects of drugs on membrane currents.