B016877 - BIOTECHNOLOGY TECHNIQUES II

Italian

Analysis of the main techniques for the detection of bacteria, fungi and viruses: techniques for the growth and phenotypic identification of these infectious agents; molecular and innovative rapid techniques

As educational material will be provided by the teachers of the slides of the lectures and protocols of the techniques applied during laboratory exercises

Knowledge and understanding: Main technical standards and innovative for the cultivation, identification and typing of microorganisms and viruses. Susceptibility testing to antibiotics and antivirals.
Applying knowledge and understanding: Ability to apply the appropriate techniques both traditional and molecular identification of microorganisms and viruses mainly involved in human infections

Laboratory safety course and medical examination

Theoretical / practical Lessons and laboratory

None

The exam consists of a written test related to the module. The written test evaluates the student's ability to apply the knowledge acquired during the course, demonstrating that he has achieved the objectives set by the specific module.
The difficulty level of the test questions corresponds to the program performed for each module. 30 points are attributed to the test if it is resolved completely and without errors; the test is considered passed if the evaluation of the test is at least 18 points. The final grade will be obtained from the average obtained from the tests of the two modules that make up the course.

Topic 1: Microbiology Laboratory: Tools and procedures for working safely in the laboratory

Topic 2: Bacteriology:
1- culture media (standard, enhanced, selective and differential) and procedures for bacterial isolation: fresh examination, isolation and culture of bacteria in soil solids and liquids; macroscopic examination bacterial colonies; Gram stain; Ziehl-Neelsen.
2- Techniques for typing bacterial biochemistry (rapid and automatic)
3- Techniques for sensitivity to antibiotics: determination of MIC (Method of dilutions in broth; Method of dilutions in agar; Kirby Bauer test (agar diffusion); automated systems.
4- Molecular biology techniques applied to bacteriology
5- Using bacteria in biotechnology

Topic 3: Mycology:
1 Illustration of the main methods of cultivation and land used for fungi
2-box colors identifying and biochemical tests for identification of yeasts
3-Using yeast in biotechnology
4-molecular techniques in the identification of fungi

Topic 4: Virology:
1-Using cell cultures for the isolation of animal viruses
2-Observation of cytopathic effects and viral inclusion bodies
3-physical and biological viral titration (method of plaques and TCID50)
4-Techniques to search for viral antigens (ELISA, WB, IFA)
5-Technical hemagglutination, hemagglutination inhibition, neutralization
6-Techniques for analysis of viral susceptibility to antivirals: phenotypic and genetic
7-Use of animal viruses and not in biotechnology
8- Bacteriophages and method of cultivation: application examples in bacterial typing
9-Molecular biology techniques in the identification and quantification of viral
10-Use of computer programs and databases for the study of genetic viral

Topic 5: Serology:
1-Main serological techniques for research antibodies specific for viral and bacterial infections