Lecture notes and articles provided during the classes
Learning Objectives
Learning of some experimental techniques for the optical investigation of living systems.
Prerequisites
Bachelor Degree
Teaching Methods
Lessons and laboratory experiences
Type of Assessment
Written report of the experimental experience and oral exam
Course program
Lectures:
Principles of linear and non-linear optical microscopy. Main detectors for optical microscopy (sCMOS, EMCCD, photomultiplier tubes, quadrant photodiodes). Chromophores and labeling of biological samples.
Introduction to the experimental setups used in laboratory. Transfer function of an optical system. Brownian motion and anomalous diffusion. Analysis of microscopy images in the spatial and temporal domains.
Laboratory:
Students will be guided in the development of a fluorescence optical microscope that will be used in the two laboratory experiences. The main properties of the microscope will be characterized, including the transfer function.
Experience I. Fluorescence microscopy of single biological molecule in living cells. Analysis of diffusive and anomalous motions and separation of active, diffusive and confined motions.
Experience II. Imaging of neuronal activity in zebrafish larvae through fluorescent molecules sensitive to calcium. Analysis of the spatio-temporal properties of the fluorescence traces.