Structure and function of proteins. Kinetic and enzymatic catalysis. Structure and function of carbohydrates. Structure and function of lipids. Structure and function of nucleic acids. The metabolism of proteins, carbohydrates, lipids, nucleic acids. Principles of metabolic regulation. Organ/tissues biochemical specialization and metabolic integration in human.
Nelson & Cox - I principi di biochimica di Lehninger - Zanichelli editore. Garrett R.H., Grisham C.M. - "BIOCHIMICA" - Ed. Piccin.
Siliprandi, Tettamanti - "BIOCHIMICA MEDICA" - Ed. Piccin.
The course aims to present to the student the current knowledge of the molecular basis of cellular life both from a structural and functional point of view, thus describing the structure and function of biological macromolecules with insights related to metabolic processes and their regulation.
The course requires knowledge of animal and plant biology, general chemistry, organic chemistry, physiology and anatomy
Lectures (72 hours)
Slides of lessons will be downloaded on server. The required password will be provided to the students during lessons.
It is strongly recommended to attend the lectures.
Office hours: every day by appointment.
Modalità di verifica apprendimento
The student will be examinated on the topics discussed during the course. In particular the knowledge, the deepening, the reasoning skills, the overall view of the Biochemistry, the clarity of the exposition, the use of a language with appropriate terminology will be considered.
Programma del corso
Amino acids. The peptide bond. Protein structure. Structure and function of myoglobin and hemoglobin. Oxygen and carbon dioxide transport. Enzymes. Classification of enzymes. Michaelis-Menten equation. Irreversible and reversible inhibitors. Allosteric enzymes. Examples of enzymatic catalysis and regulation of enzymatic activity. Soluble vitamins and coenzymes. Structure and function of carbohydrates. Structure and function of lipids. Structure and function of biological membranes. The membrane transport. Intracellular signaling. Structure and function of nucleic acids: DNA, messenger RNA, ribosomal RNA and transfer RNA. Biosynthesis, post-translational modifications and protein degradation. General concepts of Metabolism. Glycolysis. Lactic and alcoholic fermentation. Gluconeogenesis. The Cori-cycle. Glycogen synthesis and glycogenolysis. Pentose phosphate pathway. Shuttle for the transport of electrons. Pyruvate dehydrogenase. Citric acid cycle (Krebs cycle). Electron transport and oxidative phosphorylation. Coupling between electron flow and oxidative phosphorylation: mechanism of ATP-synthase. Mitochondrial transport systems. Digestion and transport of dietary lipids. Plasma lipoproteins. Fatty acids beta-oxidation. Ketogenesis and ketone bodies. Synthesis of fatty acids. Triglyceride biosynthesis. Phospholipid and cholesterol biosynthesis. Digestion and absorption of dietary proteins. Catabolism of proteins and amino acids: deamination and transamination. The urea cycle. Amino acids as precursors of biomolecules: biosynthesis of biogenic amines; biosynthesis of creatine phosphate; biosynthesis and function of glutathione; biosynthesis of heme. Biosynthesis of purine and pyrimidine nucleotides. Biosynthesis of deoxyribonucleotides. Degradation of purine and pyrimidine nucleotides. Regulation of nucleotide metabolism. Metabolic specialization of organs in humans.