As teaching material, the slides of the lessons and the protocols of the techniques applied during the laboratory exercises will be provided by the teachers
Knowledge and understanding: The aim of the course is to provide students with theoretical / practical knowledge and the ability to understand and interpret the most widespread techniques and technologies (traditional and innovative, low / medium / high productivity) in the laboratories of molecular biology.
Ability to apply knowledge and understanding: The main objective is to acquire students the ability to identify and apply the most popular techniques for the extraction of nucleic acids, their qualitative and quantitative evaluation, their amplification by polymerase chain reaction, identification of known and unknown genetic variants, evaluation of gene expression, basic analysis methods of these data, cloning, gene transfer.
Theoretical / practical lessons and laboratory exercises.
Type of Assessment
Written verification test.
Multiple choice questions.
The proposed questions and answers are aimed at assessing learning 1) knowledge of the principles of techniques and technologies presented during the lectures and 2) skills developed during laboratory exercises
-Plasmid DNA extraction by minipreps on column. Electrophoresis of plasmid DNA on agarose gel. Plating bacteria on agarose to isolate single colonies. Inoculum in liquid medium of an isolated colony.
Plasmid cutting with restriction enzyme. Control of the presence of the insert by electrophoresis on agarose gel. Detection of the insertion of single or double inserts and their directionality by analyzing an electrophoretic trace. Microscopic observation of various types of cultured mammalian cell lines (tutorial).
Results of the experiment of ectopic expression of the TK receptor. Discussion of the practical activity (frontal).
Examples of investigation using gene transfer in cultured mammalian cells: analysis of published papers (first part, frontal).
Examples of investigation using gene transfer in cultured mammalian cells: analysis of published works (second part, frontal).