Methods for molecular anthropology and paleogenetics applied to cultural heritage.
DNA extraction, amplification by PCR, DNA sequencing. Sequence analysis.
Human skeletal anatomy. Techniques for the study of human skeletal remains. Restoration. Identification of isolated bones. Age and sex determination. Identification of the main morphological features and determination of the main metrical traits. Skeletal activity patterns. Pathologies.
Chiarelli, B. Dalla Natura alla Cultura Piccin
Minozzi S. e Canci A. 2015. Archeologia dei resti umani. Dallo scavo al laboratorio. Carocci, Roma.
Davi Caramelli 2009. Antropologia Molecolare - Manuale di base. Firenze University Press
David Caramelli & Martina Lari 2004. Il DNA antico: metodi di analisi e applicazioni. Angelo Pontecorboli
Basic laboratory methods in molecular anthropology.
Skeletal anatomy. Methods of restoration of skeletal remains. Methods of study of skeletal remains.
DNA extraction methods from ancient samples, amplification by PCR, DNA Sanger sequencing. Molecular sex determination. Use of shared software for genetic data analyses.
Identification of single bones, also fragments. Assessment of age and sex from skeletal remains. Identification of activity patterns.
Skills acquired (at the end of the course):
Ability to extract, typing and sequencing DNA from ancient samples.
Technical skills for restoration of skeletal remains. Analytical skills on diagnostic morphological features.
Courses to be used as requirements (required and/or recommended)
Total hours of the course (including the time spent in attending lectures, seminars, private study, examinations, etc...): 150
Hours reserved to private study and other individual formative activities: 100
Contact hours for: Lectures (hours): 2
Contact hours for: Laboratory (hours): 48
Frequency of lectures, practice and lab:
6 hour of lessons per week.
Type of Assessment
Each student is evaluated by a written essay on the molecular procedures and by a written final essay on the specimens restored and analyzed during the course. Also an examination before specifically formed commissions.
Understanding of basic laboratory methods including DNA extraction from various substrates, DNA amplification by PCR, DNA quantification by spectrophotometry, Sanger sequencing. Molecular sex determination. Reconstruction of genealogical relationship. Use of shared genetic resources (e.g. genetic databases, software).
Basic notions of human skeletal anatomy. Lab techniques for the study of human skeletal remains. Restoration. Identification of isolated bones. Age and sex determination. Identification of the main morphological features and determination of the main metrical traits.
Skeletal activity patterns. Pathologies. Basic notions of data management. Basic notions of molecular anthropology.